Skip to main content
. 2011 May 12;7(5):e1002040. doi: 10.1371/journal.ppat.1002040

Figure 2. HMB-PP stimulated Vγ9/Vδ2 T cells induce neutrophil activation and production of pro-inflammatory mediators.

Figure 2

(A) Dose-dependent up-regulation of CD11b and shedding of CD62L by neutrophils incubated with LPS as positive control, and of neutrophils co-cultured with Vγ9/Vδ2 T cells at various ratios in the presence of HMB-PP at different concentrations. Neutrophils were analyzed after 20 hours in culture; histograms depict representative CD11b and CD62L stainings for live CD3− CD15+ neutrophils cultured under the indicated conditions. Data shown are mean fluorescence intensities (MFI) + SEM from independent experiments using three different donors. (B) Dose-dependent secretion of CXCL8 and TNF-α into the culture supernatant of neutrophils incubated with LPS as positive control, and of neutrophils co-cultured with Vγ9/Vδ2 T cells at various ratios in the presence of HMB-PP at different concentrations. Supernatants were analyzed after 20 hours by ELISA. Data shown are mean levels (pg/ml) + SEM from independent experiments using three different donors.