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. 2011 May 12;6(5):e19632. doi: 10.1371/journal.pone.0019632

Figure 6. unc-51 regulates the subcellular localization of Fas II.

Figure 6

(A, B) Localization of Fas II in the wild-type and unc-51 25/25 MBs. In wild type, Fas II (magenta) was localized only to the lobes and the distal peduncle. In unc-51 25/25 mutant MBs, Fas II was mislocalized to the calyx (yellow dashed circles) and the proximal peduncles (arrowheads). MBs were labeled with UAS-mCD8::GFP (green) driven by 201Y-GAL4. (C–H) Localization of Fas II monitored with a YFP fusion construct. Fas II::YFP transgene (green, white) was expressed by the 201Y-GAL4 driver. Counterstained with anti-Dac and anti-N-Cadherin antibodies to visualize the MB neurons (magenta). In wild type (C–E), Fas II::YFP was localized to the lobes and the distal peduncle (not shown), as was the endogenous protein. Loss of unc-51 caused mislocalization of Fas II in the calyx (G). Aberrant Fas II::YFP accumulations (arrows) were observed in the cell bodies (F) and the lobes (H). Note that the internal core was disrupted in the mutant lobes (H). (I) Quantification of the number of axonal clogs in the lobes of the wild type and unc-51−/− mutant clones. (J–Q) Cell autonomous activity of unc-51 is required for intracellular transport of Fas II and Syt1. Wild-type (J–M) and unc-51 3/3 mutant (N–Q) neuroblast clones. Clones were induced by an early first instar heat shock and labeled with UAS-mCD8::GFP driven by elav c155 (green). Fas II was mislocalized in the proximal part of the axons in all the mutant clones (13/13) (arrowheads in M). Most of the mutant clones also exhibited ectopic Syt1 accumulation (10/12) in the proximal part of the MB axons (arrowhead in P). None of the wild-type clones accumulated Fas II (0/9) or Syt1 (0/8) in the corresponding regions. CB, cell bodies; Cx, calyx (indicated by yellow dashed circles). Scale bar, 10 µm.