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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gene Ther. 2010 Dec 23;18(5):501–508. doi: 10.1038/gt.2010.173

Figure 1.

Figure 1

Expression cassettes. Cassettes were tested both in plasmid vectors in vitro and in HDAd in vitro and in vivo. Symbols: CMV = CMV promoter from pCI plasmid; i = β-globin/IgG chimeric intron from pCI; MCS = multiple cloning site; pA=SV40 poly A signal; 5U = 5′-untranslated region of rabbit IL-10 gene; 1, 2, 3, 4, 5 (yellow blocks) = rabbit IL-10 exons; 3U = 3′-untranslated region of rabbit IL-10 gene; mET-1 = native murine endothelin-1 enhancer-promoter; red arrows = ET-1 enhancer (ETE) in native position and with 3 additional ETE added as direct repeats (4XETE); ET-1 EX-A and ET-1 EX-B = 5′ and 3′ segments of mouse ET-1 exon 1 (untranslated); ET-1 intron = 5′ segment of mouse ET-1 intron 1; oPRE = optimized woodchuck hepatitis virus post-transcriptional regulatory element. Drawing is not to scale.