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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gene Ther. 2010 Dec 23;18(5):501–508. doi: 10.1038/gt.2010.173

Figure 5.

Figure 5

Enhancement of IL-10 expression and preservation of EC-specificity after addition of the optimized woodchuck hepatitis virus posttranscriptional regulatory element (oPRE). (a) EC were transduced with HDAd containing the CMV-c-IL-10 expression cassette or the 4XETE-cIL-10 cassette both without and with the oPRE. IL-10 mRNA was measured by qRT-PCR. Mean IL-10 expression for the CMV-cIL-10 cassette was assigned a value of 100%; IL-10 expression in individual wells was calculated as a percentage of this value. (b) EC or SMC were transduced with HDAd containing the CMV-cIL-10 or the 4XETE-cIL-10-oPRE cassettes (Figure 1) and IL-10 mRNA measured by qRT-PCR. For each cell type, mean IL-10 expression for the CMV-c-IL-10 group was assigned a value of 100%; IL-10 expression in individual wells was calculated as a percentage of this value. Bars in all panels are group means.