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. 2011 Apr 25;108(19):8030–8035. doi: 10.1073/pnas.1016088108

Fig. 6.

Fig. 6.

Effects of GPR41-mediated regulation of sympathetic activity on energy expenditure. (A) Effects on oxygen consumption in Gpr41−/− mice during feeding and at 48-h starvation. Measurement of oxygen consumption at 24 h after tyramine administration (100 mg/kg i.p.) (n = 5–7). (B) Body temperature of Gpr41−/− mice during feeding (n = 6). (C) Ucp1 expression in brown adipose tissue (BAT) in Gpr41−/− mice during feeding (n = 6). Internal control: 18S rRNA expression. (D) Rate of oxygen consumption in propionate and PBS administration. Oxygen consumption was measured at 40 min after propionate administration (1 g/kg i.p.) (n = 4–8). (E) Rate of oxygen consumption in β-hydroxybutyrate and PBS administration. Oxygen consumption was measured at 50 min after β-hydroxybutyrate administration (500 mg/kg i.p.) (n = 5–7). Propionate and β-hydroxybutyrate were administrated after 24 h treatment of tyramine. Hatched bars, tyramine-treated (A, D, and E). (F) Inhibitory effects of β-hydroxybutyrate on oxygen consumption. After pretreatment with β-hydroxybutyrate (500 mg/kg) for 10 min, at time 0, a bolus of propionate (1 g/kg) with or without β-hydroxybutyrate (500 mg/kg) was administered intraperitoneally (n = 8). Data were measured at time 40 min. Mice were analyzed at 14–16 wk of age. *P < 0.05; **P < 0.005.