Fig. 5.

Severe pathology in Atp11c mutant mice induced by a cholic acid-supplemented diet. Atp11c mutant and control males were fed with a cholic acid-supplemented (n = 7 and n = 5, respectively) or control diet (n = 7 and n = 5, respectively), and monitored for weight loss (A), mortality (B), and changes in plasma constituents (C). Gallbladder distention (D) and jaundice (E) as observed in littermates on a cholic acid diet for 13 d. (F) Relative expression of Cyp7a1 and Atp11c mRNA in the livers of mice after 7 d of a cholic acid-supplemented or control diet (normalized to Actb). (G) Sirius red staining of liver sections after 13 d of a cholic acid-supplemented diet. Error bars in A, C, and F represent SEM.