Fig. 3.

IECs of infected mice mainly respond to virus-induced IFN-λ. Intestinal tissue from rotavirus-infected suckling wild-type, IL28Rα^0/0, IFNAR1^0/0, and IFNAR1^0/0IL28Rα^0/0 (dKO) mice was harvested at day 4 postinfection. Paraffin-embedded samples were subjected to simultaneous staining for Mx1 (green), rotavirus antigen (white), and E-cadherin (red). Counterstaining was performed with DAPI (blue). (Right) Zoom column represents larger magnifications of the adjacent boxed areas. (Scale bars, 50 μm in first three columns; 20 μm in the zoom column.)