Fig. 5.

Mice primed with a CD8 epitope from influenza nucleoprotein, alum plus MPL, are protected from influenza A. (A) B6 mice were immunized with NP/OVA or NP/BSA either alone (closed diamonds) or with alum (open diamonds), MPL (open squares), both alum and MPL (closed squares), or the two adjuvants and PBS (closed triangles). Five to 14 wk later, the mice were infected with 150 pfu of influenza A intranasally and weighed each day. The data shown are combined from two experiments with four or five mice per group. (B) The experiments were performed as in Fig. 5A, but the numbers of D^b/NP tet⁺ cells in one lung lobe were examined on day 4. The data shown are combined from three experiments with four or five mice per group. The error bars represent SEM. (C) The experiments were performed as in Fig. 5A, but the viral titers in one lobe of the lung were measured on day 4. The data were combined from three experiments. Error bars represent SEM. (D) The experiments were performed as in Fig. 5A, but the numbers of D^b/PA tet⁺ cells in one lung lobe were examined 8 d after infection. The data are from one of two experiments with four or five mice per group. (E) B6 mice were primed with NP/OVA or NP/BSA with the indicated adjuvant, and the numbers of D^b/NP tet⁺ cells in spleens was examined 6–14 wk later. Each symbol represents a mouse, and the line indicates the group mean. The data are from three experiments with three or four mice per group.