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. 2011 Apr 25;108(19):7844–7849. doi: 10.1073/pnas.1017664108

Fig. 3.

Fig. 3.

PABP4 depletion causes anterior defects and embryonic lethality. (A) Representative photographs of stage 45–47 embryos injected with control or PABP4 morpholino ± 1 ng of PABP4-FLAG rescue mRNA. Arrows indicate abnormal or absent proctodeum. (B) Control (stage 45/46) and PABP4 morphants, which show ventral and cephalic edema (large arrowhead), absence of eye (small arrowhead), and reduced intestinal torsion (arrow). (C) Percentages of control, PABP4 morpholino, and PABP4 rescue embryos (stage 47–50) displaying the indicated phenotypes. Morphological defects include ventral and cephalic edema, abnormal head and eye development, and digestive tract defects. Data represent the average of three independent experiments, with ∼400 embryos per experimental point. (D and E) Representative photographs of stage 35–37 control embryos and embryos injected anteriorly (Ant) or posteriorly (Post) with (D) PABP4 or (E) ePABP-A (ePABP) morpholino. Arrowheads indicate abnormal head/eye development; and arrows indicate abnormal posterior development. (F) Percentages of control, PABP4, and ePABP-A morpholino embryos from D and E displaying the indicated phenotypes. Data represent the average of five (PABP4) or three (ePABP) independent experiments, with ∼120 or 90 embryos, respectively, per experimental point. (G) Polysomal profiling of stage 40–42 control and PABP4 morphants over a 10–50% sucrose gradient with a 60% cushion. A representative experiment is shown, and the positions of the 80S ribosome and polysomes are indicated.