Table 2.

Transcripts that differed between con- and heterospecifically mated females that were (i) candidate D. mojavensis/D. arizonae Acps (ii), genes from D. melanogaster male reproductive tracts, or (iii) proteins that were transferred in the ejaculate of D. melanogaster

D. moj ID	Gene	Source of transcript	Confirm§	D. moj Acp	D. mel male RT	Protein transferred in D. mel	D. mel ortholog	Conspecific mating effect	Reference
GI17858		Male	No†	Yes	Yes		CG14034‖		45, 47
GI20219		Male	Yes	Yes	Yes	Yes	CG30488		45–47
GI19546		Male	No†	Yes		Yes	CG34002		45, 46
GI20999	Dmoj/Acp11	Male	Yes	Yes			—		27
GI20988	Dmoj/Acp2	Male	Yes	Yes			—		27, 45
GI11629	Adk3	Male	Yes		Yes		CG6612		47
GI23890	ScprC	Male	Yes		Yes		CG5106	Yes	47
GI14761	rpk	Male	No¶				CG1058‖	Yes
GI11382		Male	Yes				—
GI17134		Male	Yes				CG14069	Yes
GI23381		Male		Yes	Yes	Yes	CG17097		45–47
GI23009		Male		Yes	Yes		CG10284‖		45, 47
GI16594		Female		Yes			CG1318		45
GI22128		Female		Yes			CG34215‖		45
GI13447	Cam	Female			Yes		CG8472		47
GI10528		Unknown†		Yes	Yes	Yes	CG14061		45–47
GI18622	Dmoj/Acp2b*	Unknown		Yes			—		45
GI10529		Unknown†		Yes		Yes	CG9997		45, 46
GI10530		Unknown†		Yes			—		45
GI13594		Unknown‡		Yes			CG18233‖		45
GI13596		Unknown‡		Yes			CG18233‖		45
GI20607		Unknown‡		Yes			CG4812		45
GI21941	capt	Unknown‡			Yes		CG33979		47

Transcripts were screened by RT-PCR and sequencing to determine whether they were of male or female origin.

*Previously unannotated D. mojavensis gene.

^†Did not amplify.

^‡Amplified, but problems sequencing.

^§Independent replicate experiment.

^¶Female origin in replicate experiment.

^‖Orthologous call made by BLAST to D. melanogaster genome; see Table S1.