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. 2011 Apr 25;108(19):7938–7943. doi: 10.1073/pnas.1017360108

Fig. 1.

Fig. 1.

HMW-HA costimulation induces a TR1-like phenotype in CD4+GFP/FoxP3−CD62L− effector memory T cells. (A) IL-10 production upon activation with aCD3/28 alone or in conjunction with the ECM components HMW-HA, COL, and fibrinogen (FB). (B) Effects of HMW-HA or LMW-HA treatment on IL-10 production. (C) Fold change in TH1, TH2, and TH17 cytokines levels upon HMW-HA costimulation (n ≥ 5 independent experiments each for A–C). (D) IL-10 production upon activation with aCD3/28, HMW-HA alone, or the two reagents in combination. Each condition was performed in triplicate; the image is representative of three experiments. (E) Fold change in IL-10 concentration in cell culture supernatants taken from mouse CD4+GFP/FoxP3− T cells sorted on the basis of CD62L expression and activated with or without HMW-HA (n = 6). For A, C, and E, data were normalized to the aCD3/28 condition, with this value equaling 1 for each experiment.