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. 2011 Apr 15;108(19):7950–7955. doi: 10.1073/pnas.1102454108

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Fig. 3. — CD44^hi cells display stem-like properties in vitro. (A) Mammosphere-forming ability of bulk HME or HME-flopc cells, HME-flopc-CD44^lo single-cell clones (SCCs, n = 5), HME-flopc-CD44^hi SCCs (n = 4), and CD44^hi and CD44^lo cells purified by FACS from two independent HME-flopc SCCs (FL1 and FL2). Results are mean ± SEM. (B) Representative flow cytometry plots for the markers CD44, CD24, and ESA of cells isolated from dissociated mammospheres from de novo-derived CD44^hi cells (FL1-CD44^hi and FL2-CD44^hi) and two stable HME-flopc-CD44^hi SCCs (FH1 and FH2). (C) Quantification of total CD44^lo cells, CD44^loESA⁻ and CD44^loESA⁺ populations [from (B) box P7; n = 3]. Results are mean ± SEM. (D) Frozen sections of mammospheres (5 μm) stained by immunofluorescence for myoepithelial [CD10, vimentin, and cytokeratin-14 (K14)] and luminal (K14 and MUC1) differentiation markers.