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Reconstruction and analysis of t-tubule from rabbit myocyte. (A) Three-dimensional reconstruction of t-tubule at rest with simple topology. (B) Cross-sectional area, (C) ellipticity ɛ, and (D) orientation α were determined along the length of the t-tubule. An orientation α equal to zero denotes that the minor axis of the t-tubule cross-section is parallel to the myocyte long axis.
