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. 2011 May 18;100(10):2440–2449. doi: 10.1016/j.bpj.2011.03.062

Figure 7.

Figure 7

Photobleaching and fluorescence intensity analysis of GFP-Katanin-60. (A) Representative example of three-step photobleaching event in the presence of 2 mM ATP. (B) Example of multiple photobleaching events in the presence of 2 mM AMPPNP. (C) Probability distribution of number of bleach events per complex in the presence of 2 mM ATP (white bars) and 2 mM AMPPNP (black bars). The average number of bleaching events is 4/complex for ATP; whereas the average number of bleaching events is 6/complex for AMPPNP.