Figure 4.

Overlay of EPR spectra from MSL and MTSL of Tm in solution (Tm) (gray line) and Tm on actin filament (Tm-actin₇) (dark line). (a) Spectra from MSL at residue 146. Difference spectrum was produced by subtracting 50% of the Tm spectrum from Tm-actin₇ spectrum. (b) Spectra from MSL at residue 160. The difference spectrum was produced by subtracting 40% of Tm spectrum from Tm-actin₇ spectrum. (c) Spectra from MSL at residue 230. The difference spectrum was produced by subtracting 78% of Tm spectrum from Tm-actin₇ spectrum. (d) Spectra from MSL at residue 279. The difference spectrum was produced by subtracting 60% of Tm-actin₇ spectrum from Tm spectrum. (e) Spectra from MSL at residue 13. (f) Spectra from MSL at residue 271. (g) Spectra from MTSL at residue 271.