Figure 6.

OLIG2^S147A Promotes OL Fate Specification but Represses MN Fate in Cultured P19 Cells

(A–D) MN induction: addition of SHHAg1.2 agonist and RA induced aggregated P19 cells to express the MN marker HB9 (B and B′). This induction was accentuated in stable cell line P19-OLIG2^WT (C and C′) but repressed in P19-OLIG2^S147A (D and D′).

(E–H) OL induction: SHHAg1.2 and RA also induced aggregated P19 cells to express OLP marker NG2 (F and F′). This induction was slightly amplified in the P19-OLIG2^WT line (G and G′) but dramatically magnified in P19-OLIG2^S147A (H and H′).

(I, I′, and I″) The NG2-positive cells (green) that were induced in P19-OLIG2^S147A cultures also expressed SOX10 (red), confirming them as OL lineage cells. Cell nuclei were counterstained with DAPI (blue).

(J) Quantification of HB9 and NG2-inducing activity.

Scale bar, 50 μm (A–H′ and I–I″). See also Figure S5.