Figure 6.
Nap1 Stabilizes the (H3-H4)2 Tetramer
(A) Histones carrying nontetramerizing mutations can form bona fide nucleosomes. The positions of DNA and nucleosomes (Nuc.) are indicated. The efficiency of nucleosome assembly, quantified by the proportion of DNA incorporated over total DNA present, is displayed at the bottom of the gel as a fraction of the wild-type.
(B) Nap1 can partially restore tetramerization of H3 C110E-containing dimers. H3 K115C crosslinking mutation was introduced into the C110E construct and crosslinked alone and in the presence of Nap1. Crosslinking efficiency of the wild-type (containing H3 K115C) is shown for comparison.
(C) Quantification of crosslinking efficiency shown in (B). The average and standard deviation of three independent experiments are shown.
See also Figure S5.