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Epac and PKA synergise to induce stellate morphology. Asynchronous VSMC were stimulated with 200 μM BNZ, CPT or BNZ plus CPT as indicated. A, Phase contrast images showing cell morphology. B, Phalloidin staining for F-actin. C, Paxillin staining representing focal adhesions, D, the area of which was quantified using NIH ImageJ, n = 3. * indicates p < 0.05 versus control.
