Table 2.
Effect of surgery, caerulein-induced mild pancreatitis and taurocholate-induced severe pancreatitis on mitochondrial function (lung, jejunum and pancreas)
| Tissue | Saline control | Caerulein-induced mild pancreatitis | Sham surgery | Taurocholate-induced severe pancreatitis |
|---|---|---|---|---|
| Lung | ||||
| LEAK | 10.8 ± 0.9b | 8.1 ± 1.2 | 4.2 ± 0.3b | 4.8 ± 0.4 |
| OXP-I | 71.5 ± 7.8a,b | 42.8 ± 5.1a | 27.3 ± 1.7b | 26.8 ± 2.5 |
| OXP-I,II | 98.7 ± 11.2a,b | 61.6 ± 7.4a | 38.7 ± 3.0b | 38.0 ± 3.7 |
| ETS | 107.4 ± 14.9b | 67.7 ± 8.3 | 41.7 ± 4.2b | 42.1 ± 3.4 |
| ETS-II | 55.8 ± 9.5 | 41.9 ± 5.1 | 26.0 ± 2.2 | 28.8 ± 3.8 |
| CCO | 144.4 ± 22.9a,b | 73.6 ± 10.5a | 56.6 ± 5.0b | 59.3 ± 5.6 |
| CCOc | 199.7 ± 27.4b | 160.2 ± 43.7 | 86.8 ± 5.4b | 86.6 ± 12.1 |
| OXP-I,II/ OXP-I | 1.4 ± 0.0 | 1.4 ± 0.0 | 1.4 ± 0.0 | 1.4 ± 0.0 |
| ACR | 6.6 ± 0.4 | 5.6 ± 0.5 | 6.9 ± 0.8 | 5.6 ± 0.1 |
| Jejunum | ||||
| LEAK | 22.2 ± 3.7 | 18.8 ± 2.2 | 24.5 ± 1.8 | 13.7 ± 3.7 |
| OXP-I | 123.1 ± 21.3 | 98.2 ± 12.7 | 117.1 ± 8.8c | 44.4 ± 10.1c |
| OXP-I,II | 212.1 ± 37.2 | 202.8 ± 26.8 | 200.6 ± 52.9c | 73.0 ± 17.4c |
| ETS | 171.4 ± 31.2 | 183.0 ± 31.6 | 158.2 ± 20.6c | 53.7 ± 12.7c |
| ETS-II | 115.0 ± 21.5 | 110.5 ± 20.3 | 95.1 ± 14.4c | 37.9 ± 8.7c |
| CCO | 174.2 ± 21.1 | 194.1 ± 20.0 | 182.2 ± 16.3 | 132.4 ± 32.7 |
| CCOc | 337.7 ± 41.7 | 297.5 ± 30.8 | 353.2 ± 22.8 | 269.0 ± 58.8 |
| OXP-I,II/ OXP-I | 1.7 ± 0.1a | 2.1 ± 0.0a | 1.7 ± 0.1 | 1.7 ± 0.1 |
| ACR | 5.4 ± 0.48 | 5.2 ± 0.3 | 5.0 ± 0.5 | 4.4 ± 0.6 |
| Pancreas head | ||||
| LEAK | 31.9 ± 6.2b | 21.0 ± 2.9 | 11.4 ± 2.3b,c | 3.5 ± 0.5c |
| OXP-I | 137.0 ± 19.7 | 73.3 ± 8.4 | 57.6 ± 13.4c | 9.3 ± 5.2c |
| OXP-I,II | 240.3 ± 33.0 | 192.7 ± 24.1 | 134.0 ± 24.1c | 46.1 ± 11.3c |
| ETS | 232.5 ± 32.8 | 198.5 ± 25.0 | 139.0 ± 24.0c | 47.6 ± 12.7c |
| ETS-II | 177.9 ± 24.7 | 163.6 ± 20.8 | 111.4 ± 18.8 | 45.8 ± 11.8 |
| CCO | 411.1 ± 75.9 | 406.8 ± 86.2 | 249.5 ± 30.1 | 230.7 ± 64.7 |
| CCOc | 597.8 ± 108.7 | 541.8 ± 108.3 | 362.5 ± 61.8 | 324.9 ± 77.0 |
| OXP-I,II/ OXP-I | 1.8 ± 0.1a,b | 2.6 ± 0.1a | 2.6 ± 0.2b | 6.8 ± 1.7 |
| ACR | 4.6 ± 0.3 | 4.1 ± 0.9 | 5.2 ± 0.8 | 3.6 ± 2.1 |
| Pancreas tail | ||||
| LEAK | 40.0 ± 2.3a,b | 19.0 ± 2.6a | 18.2 ± 4.4b | 7.5 ± 1.4 |
| OXP-I | 146.7 ± 12.3a,b | 75.7 ± 12.6a | 70.9 ± 19.0b | 33.5 ± 6.8 |
| OXP-I,II | 247.1 ± 16.5b | 180.4 ± 27.6 | 140.4 ± 30.7b | 90.9 ± 12.1 |
| ETS | 240.3 ± 15.3a | 183.0 ± 27.5a | 139.4 ± 30.7 | 94.5 ± 13.4 |
| ETS-II | 177.3 ± 9.5 | 146.2 ± 20.9 | 108.3 ± 23.6 | 82.5 ± 12.3 |
| CCO | 313.5 ± 21.0 | 265.9 ± 37.9 | 219.1 ± 42.4 | 221.2 ± 35.6 |
| CCOc | 465.8 ± 28.6 | 441.4 ± 93.2 | 332.4 ± 87.4 | 307.7 ± 41.7 |
| OXP-I,II/ OXP-I | 1.7 ± 0.1a,b | 2.6 ± 0.4a | 2.3 ± 0.3b | 3.0 ± 0.4 |
| ACR | 3.7 ± 0.3 | 3.9 ± 0.5 | 3.9 ± 0.5 | 4.8 ± 0.8 |
Units are pmols O2/mg protein/second. LEAK: State 2 respiration with glutamate and malate. GM3: State 3 respiration with addition of adenosine diphosphate (ADP). Units are pmols O2/mg wet weight/second. OXP-I: State 3 respiration with addition of succinate. ETS: uncoupled respiration with addition of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP). ETS-II: addition of rotenone. CCO: addition of tetramethyl-p-phenylenediamine (TMPD)/ascorbate. CCOc addition of cytochrome C. OXP-I,II/OXP-II: provides a measure of complex I contribution to respiration. ACR: acceptor control ratio. Like letters represent significant differences between those groups (P < 0.05, Bonferroni corrected) whereby
Caerulein pancreatitis vs. saline control
Saline control vs. sham surgery
Sham surgery vs. taurocholate pancreatitis.