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Mutation of putative miRNA binding sites in the egl-5 3’UTR results in ectopic seam cell expression. A. V6CRE-egl-5-3’REG is not expressed in the seam in the midbody. B-F. Examples of ectopic expression patterns of several transgenes with mutated miRNA target sequences (see Fig. 2). Expression is shown in (B) the midbody seam of an L3 male; (C) posterior neurons of the L1 hermaphrodite; (D) hermaphrodite V6.p cell; (E) ray 7 and 9 precursor descendants of T seam cell in an L4 male.
