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Expression of Ccng1 and inhibition of apoptosis. (a–d) In situ hybridization for Ccng1 in whole (a,b) and sectioned (c,d) E8.5 embryos. (e,f) TUNEL staining (green) for apoptosis in E8.75 Tcof1+/+ and Tcof1+/− littermates. (g,h) Intraperitoneal injection of pregnant dams with pifithrin-α in wild-type and Tcof1+/− embryos. Scale bars, 150 μm (a,b,e–g); 40 μm (c,d).
