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Analyses of apoptosis and ribosome biogenesis. (a–c) Immunostaining of sections of E9.0–E9.5 embryos for caspase3 activity (orange) in wild-type Tcof1+/+ embryos (a), Tcof1+/− mutants (b) and in Tcof1+/−Trp53+/− littermates (c). (d–f) Immunostaining of sections of E8.5 embryos with the mature 28S rRNA-specific antibody Y10b (red) in Tcof1+/+ embryos (d), Tcof1+/− mutants (e) and in Tcof1+/− Trp53+/− embryo littermates (f). Scale bar, 50 μm.
