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Double-staining immunohistochemistry of brain sections identifies the cell types that express tissue plasminogen activator (tPA) in the ischemic boundary zone. tPA was colocalized with neuronal marker MAP2 (A), astrocytic marker glial fibrillary acidic protein (B), microglial marker IB4 (C), and endothelial cell marker von Willebrand factor (D). *P<0.05 vs wild-type control. Scale bars: 25 µm.
