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. Author manuscript; available in PMC: 2011 May 13.
Published in final edited form as: Methods Mol Biol. 2008;461:47–70. doi: 10.1007/978-1-60327-483-8_6

Fig. 4.

Fig. 4

Making grafting pipets. A glass bead is attached to the tip of the heating filament of the microforge. The bead can be heated and cooled instantly by switching on and off the current of the heating filament. The current is adjusted to yield the optimal heating appropriate to the thickness and the thermal property of the glass capillary. A, B The heated glass bead contacts the shaft of the pipet at the position that gives the desired internal diameter. C Melting the capillary wall into the glass bead. D The resultant break in the pipet after the bead cools down and retracts when the current is switched off. This produces a pipet of the desired internal diameter and an even tip.