Skip to main content
. Author manuscript; available in PMC: 2011 May 13.
Published in final edited form as: Methods Mol Biol. 2008;461:47–70. doi: 10.1007/978-1-60327-483-8_6

Fig. 5.

Fig. 5

A The manipulation chamber used for manipulating 6.5-d embryos in hanging drops. The base of the manipulation chamber is made of glass of good optical quality supplied by an optometrist. The dimension of the glass strips supporting the cover slip is 3 × 3 × 30 mm. They are set between 4 and 6 mm from the edge. Two drops of solution are placed on the cover slip approximately 1 cm apart, then the coverslip is inverted over the paraffin oil-filled chamber: hp, holding pipet; ip, injection pipet. B The petri dish setup used for manipulating 7.5- and 8.5-d embryos. Two drops are placed approximately 1 cm apart in the center of the dish then covered with paraffin oil. Angled pipets are used: hp, holding pipet; ip, injection pipet.