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. Author manuscript; available in PMC: 2011 May 13.
Published in final edited form as: Methods Mol Biol. 2008;461:47–70. doi: 10.1007/978-1-60327-483-8_6

Table 1.

The Composition of PB1 Medium for Handling Embryos and Tissue Fragments

Stock solution (g/500 mL) Volume (mL) to add to make 100 mL solution
NaCl (4.5) 65.8
KCl (5.75) 1.8
Na2HPO4 (10.93) 5.2
KH2PO4 (10.5) 0.9
CaCL2·2H2O (8.1) 0.8
MgCl2·6H2O (16.55) 0.3
Na pyruvatea 21.4
Phenol reda 0.8
Penicillina 0.3
Distilled H2O 0.27
a

See text for method of preparation.