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. 2011 Mar 11;286(20):17435–17444. doi: 10.1074/jbc.M110.137273

FIGURE 2.

FIGURE 2.

Stretch increased production of low molecular mass HA. A, quantitative RT-PCR of HA synthase genes (has) 1–3. has1 gene expression is significantly decreased with TGF-β1 or stretch compared with static culture. has2 gene expression is significantly decreased in the stretched AT2 cells compared with all other groups. has3 gene expression is significantly increased in static + TGF-β1 and stretched AT2 compared with static treated AT2 cells. B, agarose gel electrophoresis of AT2 supernatant. Lane 1, high molecular mass HA ladder; lane 2, low molecular mass HA ladder; lane 3, static culture supernatant; lane 4, stretch supernatant; lane 5, static supernatant with hyaluronidase treatment; lane 6, stretch supernatant with hyaluronidase treatment. C, immunohistochemistry for HA of statically cultured AT2 (left) and stretched AT2 (right). Nuclei are stained with DAPI. Scale bar, 20 μm. D, ELISA for HA concentration (normalized to protein concentrations) showing statistically significant higher HA in the stretched supernatant. Data are represented as means ± S.E. (error bars), n = 3/group. ***, p < 0.001 ANOVA with Tukey's post hoc test.