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. 2011 Mar 11;286(20):17435–17444. doi: 10.1074/jbc.M110.137273

FIGURE 5.

FIGURE 5.

Cells deficient in the innate immune adaptor molecule MyD88 do not undergo stretch-induced EMT. A, immunoprecipitation with CD44 or IgG control and probed with an antibody to TLR-4 or CD44 as control. Stretch or TGF-β1 causes co-immunoprecipitation of TLR-4 and CD44. B, gene expression of E-cadherin significantly decreased with stretch in wild-type cells but not in MyD88−/− cells. C, gene expression of vimentin not significantly increased in stretched MyD88−/− cells. D, gene expression of α-SMA significantly increased with stretch in wild-type cells but not in MyD88−/− cells. E, representative Western blot of AT2 from wild-type and MyD88−/− static and stretched cells. Data are represented as means ± S.E. (error bars), n = 4/group. *, p < 0.05; **, p < 0.01 ANOVA with Tukey's post hoc test.