TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| S. meliloti strains | ||
| Rm1021 | Smr derivative of SU47 | Ref. 36 |
| acpXL | Rm1021 acpXL::pk18mobGII Nmr | Ref. 9 |
| lpxXL | Rm1021 lpxXL::pJH104 Nmr Hmr | Ref. 9 |
| adhA2XL | Rm1021 adhA2XL::pk18mobGII Nmr | This study |
| fabF1XL | Rm1021 fabF1XL::pk18mobGII Nmr | This study |
| fabF2XL | Rm1021 fabF2XL::pk18mobGII Nmr | This study |
| fabZXL | Rm1021 fabZXL::pk18mobGII Nmr | This study |
| msbA2 | Rm1021 msbA2::pJH104 Nmr | Ref. 25 |
| E. coli strains | ||
| DH5α | supE44ΔlacU169 (w80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Bethesda Research Laboratories |
| MT616 | MM294A recA56 (pRK600) Cmr | Ref. 17 |
| Plasmids | ||
| pRK600 | pRK2013 npt::Tn9 Cmr | Ref. 17 |
| pk18mobGII | mob lacZα gusA Kmr | Ref. 16 |
| pRF771 | pTE3 with an alternative polylinker TcR | Ref. 18 |
| pSmfabZXL | pRF771 carrying fabZXL with a 31 bp upstream of the start codon of the gene TcR | This study |
| pSmfabF1XL | pRF771 carrying fabF2XL with a 54 bp upstream of the start codon of the gene TcR | This study |
| pSmfabF2XL | pRF771 carrying fabF1XL and 31 bp upstream of the start codon of the gene TcR | This study |
| pSmadhA2XL | pRF771 carrying adhA2XL and 31 bp upstream of the start codon of the gene TcR | This study |
| pSmacpXL | pRF771 carrying acpXL and 40 bp upstream of the start codon of the gene TcR | This study |
| pSmlpxXL | pRF771 carrying IpxXL and 33 bp upstream of the start codon of the gene TcR | This study |