TABLE 2.
Primers
Restriction enzyme recognition sites are underlined.
| Primer name | Sequence (5′–3′) |
|---|---|
| pkX_DS_R | CGAAAGGGGGATGTGCTGC |
| smc04278_−33_PstI_F | TCTTAGCTGCAGATAGGGTTCGCCAAGACTC |
| smc04278_stop_XbaI_R | CGTACGTCTAGACTCAACCCGCTTTTGC |
| smc04270_+78_EcoRI_F | GATTACGAATTCCGAGGTGACGCTGCGGG |
| smc04270_+451_HindIII_R | AGTGCCAAGCTTCGAAGGTGACCGGCGCCA |
| smc04270_−270_F | CGCCAGCCTTGTCATGAC |
| smc04270_−35_PstI_F | GCTTGGCTGCAGCTCGCGCGACAACGACATATG |
| smc04270_stop_XbaI_R | CGTACGTCTAGATCAATCCATCCGCAACACGATC |
| smc04273_+33_PstI_F | GTCGACCTGCAGTCCGATCGTTGCCGTC |
| smc04273_+406_HindIII_R | AGTGCCAAGCTTGGAACCGATTATAGGCGTCG |
| smc04273_−155_F | CGCTCTCGCTCGGACAC |
| smc04273_−31_NsiI_F | GTGATCATGCATCGTGCTTGCCGCGGAAT |
| smc04273_+1307_XbaI_R | CGTACGTCTAGACAGAGGGGGCCTTTTTCTTCCAC |
| smc04275_+36_PstI_F | GTCGACCTGCAGGGTAGGCATCGTGACGAGC |
| smc04275_+446_HindIII_R | AGTGCCAAGCTTGCCAGAAACAGCGTCGG |
| smc04275_−126_F | GATATGCGATGCTCAGCTG |
| smc04275_−54_PstI_F | GCTTGGCTGCAGGCGTGCGGAAGAACTGGG |
| smc04275_stop_XbaI_R | CGTACGTCTAGATTATTCCGCGGCAAGCA |
| smc04277_+36_PstI_F | ATCTACCTGCAGCGAAACCGTCGATCTG |
| smc04277_+204_HindIII_R | AGTGCCAAGCTTGAATTTCGTCGCGGCC |
| smc04277_−241_F | CGAGGAGTACTTCGTGCTGA |
| smc04277_−31_PstI_F | GCTTGGCTGCAGCTTGAGCGCGAAACGAATG |
| smc04277_stop_XbaI_R | CGTACGTCTAGATCACTTCTCCGAACCGGC |
| smc04268_−40_PstI_F | TCTTAGCTGCAGAGTCGCGCCAGATCTTC |
| smc04268_stop_XbaI_R | CGTACGTCTAGATCAAAGAGACCGCTTGATG |
| smc04273_+352_R | AAGCTCGAAACGGGCATT |
| smc04273_+960_F | CACGCCCGAAAACGAC |
| smc04270_+235_R | GGGAGCACATTGGCGA |
| smc04270_+698_F | AGGGCGTCGATGTCGTC |
| smc4268_+149_R | GCAACGCGATCCATGAAG |
| smc04275_+188_R | CGGAATCTGCTGCGACC |
| 8_F | AGAGTTTGATCCTGGCTCAG |
| 1492_R | GGTTACCTTGTTACGACTT |