TABLE 3.
Fatty acid composition of the mutants in the acpXL-lpxXL cluster
| Fatty acyl residue | EI/MS detector response (percentage of area)a |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rm1021 | acpXL | fabZXL | fabZXL + pSmfabZXL | fabF2XL | fabF2XL + pSmfabF2XL | fabF1XL | fabF1XL + pSmfabF1XL | adhA2XL | adhA2XL + pSmadhA2XL | |
| VLCFA | ||||||||||
| 27OH-C28:0 | 25.6 | ND | TR | 25.2 | ND | 8.5 | ND | 29.9 | ND | 37.8 |
| 29OH-C30:0 | 5.4 | ND | 1.7 | 7.3 | ND | 9.7 | ND | 6.0 | ND | 4.8 |
| 25OH-C26:0 | ND | ND | TR | ND | ND | 1.5 | ND | ND | ND | ND |
| Non-hydroxylated fatty acids | ||||||||||
| C18:1 | TR | 17.5 | 13.3 | 3.1 | 19.9 | 7.1 | 17.0 | 3.3 | 30.2 | 10.3 |
| C16:0 | ND | 4.0 | 2.0 | ND | 3.7 | TR | 2.2 | ND | 3.7 | TR |
| C18:0 | 2.0 | 4.8 | 6.7 | TR | 7.5 | 3.4 | 9.1 | 2.2 | 10.0 | 2.7 |
| Hydroxylated fatty acids | ||||||||||
| 3OH-C14:0 | 21.7 | 34.7 | 32.9 | 22.2 | 29.5 | 25.6 | 34.7 | 23.2 | 14.9 | 17.9 |
| 3OH-C16:0 | 11.3 | 6.8 | 6.6 | 5.5 | 6.4 | 7.6 | 7.3 | 5.1 | 5.7 | 3.8 |
| 3OH-C18:0 | 28.8 | 28.8 | 33.2 | 30.9 | 30.0 | 31.0 | 27.7 | 27.1 | 31.7 | 19.2 |
| 3OH-C18:1 | 5.2 | 3.4 | 3.6 | 5.9 | 3.0 | 5.5 | 2.2 | 3.3 | 3.8 | 3.6 |
a ND, not detected; TR, trace amount detected (not included in the area percentage calculation); 3OH-C17:0 and 3OH-C19:1 were found in trace amounts or not detected in all samples.