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Thermal unfolding behavior of the coiled-coil/collagen constructs. A, V-CL (○), CL (◊), CC-CL (□), CL-CC (△), and CC-CL-CC (▿) at 20 μm peptide/protein in PBS, pH 7.0. CC-CL (B) and BS-3pCC4, i.e. CC alone (C), in the presence of guanidine HCl at 0 m (□), 1.0 m(△), 2.0 m (▿), and 3.0 m (◊). Tm of coiled coil denaturation in 3 m guanidine HCl, CC-CL (69 °C, B (◊)) and BS-3pCC4 (55 °C, C (◊)).
