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. 2011 Mar 31;286(20):17521–17529. doi: 10.1074/jbc.M111.229658

FIGURE 4.

FIGURE 4.

Translational positions on 216-bp 5 S flap-out and flap-in nucleosomes. Nucleosomes were reconstituted on 215-bp templates as described in the text, and translational positions were analyzed on 5% native polyacrylamide gels. A, schematic of flap-out (FO) and flap-in (FI) nucleosome templates and translational positions of nucleosome cores as determined by restriction enzyme digestion analysis in B. B, restriction enzyme digestion analysis of FO and FI nucleosomes. Lane 1, naked DNA; lane 2, template reconstituted with H3/H4 tetramer only; lanes 3 and 4, 9 and 10, FO and FI nucleosomes, respectively; lanes 5–8 and 11 and 12, FO and FI nucleosomes digested with Bam H1, BbvI, or RsaI, as indicated. Filled arrowheads indicate the nucleosome translational positions in undigested nucleosomes, the open arrowhead indicates the position of tetramer-DNA complex, and the brackets indicate subnucleosomal complexes.