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. 2011 Mar 13;286(20):17672–17681. doi: 10.1074/jbc.M111.236612

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Inhibition of DDR1 suppresses tumorigenicity in vitro and in vivo. A, expression of DDR1 after DDR1 knockdown. P, parental HCT 116 cell lysate; C1, pBabe-shRNA vector control; C2, pBabe-luciferase control; #1, shDDR1-1 construct; and #2, shDDR1-2 construct. B, in vitro tumorigenesis assay. Clonogenic assay/colony-forming assay and soft agar assay of DDR1 knockdown HCT116 cells are shown. C, DDR1 knockdown suppresses tumor formation in xenograft model. Athymic nude mice were injected with 2 × 10⁶ cells of DDR1 shRNA knockdown HCT116 human colon cancer cells or control knockdown cells (luciferase-shRNA). Two independent DDR1 shRNA pooled clones (1 and 2, two different siRNA hairpin sequences described under “Experimental Procedures”) were used for studies.