FIGURE 2.

Effect of metalloprotease and protein kinase C inhibitors on TGFα and NRG release in MEF cells. Shown are trend lines of % control red:green fluorescent ratio as determined by FACS. MEF cells were either control-treated or pretreated with the indicated inhibitor. Subsequently, cells were either left unstimulated (No stimulation) or stimulated with one of the indicated stimuli. LPA and IM do not induce NRG cleavage in MEF cells. Solid line, control-treated cells; dashed line, metalloprotease inhibitor BB94 (10 μm); dotted line, classical PKC inhibitor bisindolylmaleimide (1 μm); dotted-dashed line, PKCζ pseudosubstrate inhibitor (10 μm); TPA, phorbol ester; sorbitol 400 mosm, hypertonic stress, 400 mosm gradient; LPA, lysophosphatidic acid (20 μm); IM, ionomycin (2.5 μm). Data are from at least three independent experiments performed in triplicate.