TABLE 1.

Comparison of pro-EGF ligand cleavage in wild type MEFs and wild type MLE cells

We previously published data examining the cleavage of pro-TGF-α, pro-NRG, and pro-HB-EGF in mouse lung epithelial cells using the same reporter assay and the same cleavage stimuli (11). Here, we compare these data to our current dataset in MEF cells at points of maximal cleavage, using % control of the red:green fluorescent ratio. Data are from at least three independent experiments performed in triplicate.

Stimulus	% Control of red:green fluorescence ratio at point of maximal cleavage
	TGF-α at 30 min		NRG at 30 min		HB-EGF at 15 min (at 30 min)
	MEF cells	MLE cells	MEF cells	MLE cells	MEF cells	MLE cells
TPA 1 μm	75	50	40	30	15 (15)	25 (20)
Sorbitol 400 mosm	75	50	50	30	15 (20)	25 (50)
LPA 20 μm	30	30	0	25	25 (0)	0 (0)