TABLE 3.

Summary of pro-EGF ligand cleavage in MEFs

EGF reporter ligand cleavage was compared at points of maximal cleavage based on the results shown in Fig. 3. Maximal cleavage of TGF-α and NRG occurred at approximately 30 min, and maximal cleavage of the HB-EGF substrate was observed at approximately 15 min. Cleavage is reported as % control. Negative values indicate an accumulation rather than cleavage of the substrate. ∅ = lack of cleavage.

Stimulus	ADAM	% control of red:green fluorescence ratio at maximal cleavage
		TGF-α at 30 min	NRG at 30 min	HB-EGF at 15 min
No stimulation	WT	∅	∅	∅
	9−/−	−20	−20	−20
	10−/−	20	10	−10
	17−/−	10	10	−10
TPA 1 μm	WT	80	40	20
	9−/−	80	∅	20
	10−/−	90	50	∅
	17−/−	∅	30	∅
Sorbitol 400 mo sm	WT	80	50	20
	9−/−	80	∅	20
	10−/−	80	40	∅
	17−/−	75	∅	20
LPA 20 μm	WT	30	10	20
	9−/−	40	∅	20
	10−/−	60	40	20
	17−/−	∅	∅	∅
Ionomycin 2.5 μm	WT	60	10	10
	9−/−	50	∅	10
	10−/−	60	40	∅
	17−/−	∅	30	∅