TABLE 1.
Primers for real-time RT-PCR
| Genes | Oligonucleotide/probe | Sequences (5′ to 3′)a |
|---|---|---|
| 18 S rRNA | 18S_F | TTCGAGGCCCTGTAATTGGA |
| 18S_R | GCAGCAACTTTAATATACGCTATTGG | |
| 18S_P | AGTCCACTTTAAATCCTT | |
| Alkaline phosphatase | AP_F | ATGCCCTGGAGCTTCAGAAG |
| AP_R | TGGTGGAGCTGACCCTTGAG | |
| AP_P | ACG T+GG +CT+A A+GA +AT+G T+CA +TC | |
| Bone sialoprotein 2 | BSPII_F | AGAGGAAGCAATCACCAAAATGA |
| BSPII_R | TTGAGAAAGCACAGGCCATTC | |
| BSPII_P | ct g+Ct +T+Ta a+Tt +Ttg +Ct+C agc | |
| Osteopontin | OP_F | ACATCACCTCACACATGGAAAGC |
| OP_R | GCTGACTCGTTTCATAACTGTCCT | |
| OP_P | ctt c+Tg +At+T ggg +Ac+A gc+C gt | |
| RUNX2 | RX_F | AGGCATGTCCCTCGGTATGTC |
| RX_R | GAAGGGTCCACTCTGGCTTTG | |
| RX_P | aca c+Ct a+Cc tg+C ca+C ca+C cc |
Plus signs designate LNA nucleotides incorporated during probe design.