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Decreased ZO-1 content in MKK4cko-TAC mice. A, immunoblot analyses of ZO-1 expression show a moderate but significant decrease in the MKK4cko-TAC heart. The ratios of ZO-1/tubulin are expressed in the bar graphs. B, immunohistochemical staining for ZO-1 confirmed decreased ZO-1 in the mutants (scale bar, 5 μm). Data are mean ± S.E (n = 6 per group).
