TABLE 1.

HPLC running conditions for peptide separation

Protease	Gradient	Time	% B
		min
SUATM/trypsin	Phase A: 5% acetonitrile/0.1% TFA
	Phase B: 80% acetonitrile/0.1% TFA	0	5
		15	5
		115	45
		117	80
		120	80
		122	5
		135	5
SUATM/chymotripsin	Phase A: 5% acetonitrile/0.1% TFA
	Phase B: 80% acetonitrile/0.1% TFA	0	2
		5	2
		10	8
		59	30
		69	80
		72	80
		73	2
		78	2