FIGURE 10.

Spatial segregation of annexins A1 and A6 plasmalemmal translocations in primary smooth muscle cells. Primary smooth muscle cells derived from human myometrium were double-transfected with annexin A6 (AnxA6)-CFP and annexin A1 (AnxA1)-YFP and treated with SLO (100 ng/ml) at time point = 0. The intracellular dynamics of two annexins were analyzed by confocal microscopy. Two different focal planes of the same cell are shown: (− SLO), before SLO was added to the cells; (+ SLO), 10 min after SLO addition. Arrows point to the regions of the plasmalemma at which the segregation of the annexins occurs. Scale bar, 10 μm.