Microvesicles containing different annexins are shed by SLO-perforated cells. HEK 293 cells double-transfected with annexin A6 (AnxA6)-YFP and annexin A1 (AnxA1)-CFP were treated with SLO (100 ng/ml) at time point = 0. As SLO permeabilization and ensuing plasmalemmal repair progress, the microvesicles containing either both annexins (Region of interest (ROI) 1) or individual annexins (Regions of interest (ROI) 2 and 3; white arrows, annexin A1, yellow arrows, annexin A6) appear in the extracellular milieu. Scale bar, 10 μm.