FIGURE 2.
Nogo-22 kDa is a potent inhibitory region of Nogo-A. A, schematic of the human Nogo-A protein illustrating the 22-kDa C-terminal region which contains three NgR1- and PirB-interacting domains. B, Coomassie-stained SDS-PAGE gel showing that purified Nogo-22 kDa migrates as two bands near the expected 22-kDa size. C, size exclusion chromatography of purified Nogo-22 reveals a major peak corresponding to a molecular weight of ∼80 kDa. Approximate elution positions for protein standards are indicated. D, E13 chick DRG growth cone collapse assay showing that Nogo-22 is a >10-fold more potent growth cone-collapsing molecule than Nogo-66. A-24, Nogo-A-24; 66, Nogo-66; C39, Nogo-C39; Vo, void volume; Vt, total volume.