TABLE 2.

Summary of effect of TRAK DN on mitochondrial dynamics in axons of hippocampal pyramidal neurons

Hippocampal neurons were transfected at 4 DIV with either pDsRed1-Mito, pDsRed1-Mito + pIRES-hrGFP-1a, or pDsRed1-Mito + pIRES-GFPTRAK2 DN. At 6 DIV, co-transfected cells were identified, mitochondrial dynamics were imaged by confocal microscopy, and the results were analyzed using Volocity software all as described under “Experimental Procedures.” Oscillating mitochondria are defined as those that are displaced >2 μm from one site. Values are the means ± S.E. from n = 33 images from n = 23 neurons for pDsRed1-Mito (295 mitochondria imaged), n = 18 images from n = 14 neurons for pDsRed1-Mito + pIRES-hrGFP-1a transfections (267 mitochondria imaged), and n = 33 images from n = 21 neurons for pDsRed1-Mito + pIRES-GFPTRAK2 DN (406 mitochondria imaged) for n = 4 independent transfection experiments. Statistical significances were obtained using the Students t test.

Clones transfected	Mitochondrial density	Velocity	Fusion events/10 mitochondria	Fission events/10 mitochondria	Oscillating mitochondria	Stationary mitochondria	Mobile mitochondria	Anterograde mitochondria	Retrograde mitochondria
	μm−1	μm/s			%	%	%	%	%
pDsRed1-Mito	0.18 ± 0.03	0.46 ± 0.17	1.1	1.0	1 ± 1	68 ± 4	32 ± 4	17 ± 3	14 ± 3
pDsRed1-Mito pIRES-GFP	0.16 ± 0.03	0.66 ± 0.25	0.9	0.9	2 ± 2	60 ± 10	40 ± 10	19 ± 2	19 ± 4
pDsRed1-Mito pIRES-FPTRAK2 DN	0.18 ± 0.03	0.48 ± 0.18	0.6a	0.5a	1 ± 1	89 ± 4b	11 ± 4b	4 ± 2c	7 ± 2a

^a p < 0.05.

^b p < 0.001.

^c p < 0.0005.