FIGURE 5.

The interaction between PTEN and its interactors in PAP is confirmed by co-precipitation. Cell extracts from U87 FLAP, FLAP PTEN WT, and FLAP PTEN G20E cells were incubated with a FLAG beads, and precipitates were analyzed with the indicated antibodies: immunoblot with anti-USP7 and anti-IQGAP1 (A) and anti-CALU (D). Lysates from HEK293T and LN 229 cells were incubated with anti-PTEN and mouse IgG-conjugated beads. The co-precipitates were detected with anti-USP7 (B) and anti-IQGAP1 (C) antibodies. GFP-trap beads were incubated with lysates of HEK293T transfected the expression plasmids of FLAP, FLAP PTEN WT, and FLAP PTEN G20E with V5-tagged NUDT16L1. The precipitates were detected with V5 and FLAG antibodies (E). IP, immunoprecipitation; WCL, whole cell lysate.