TABLE 1.
Prioritized PTEN interactors in PAP approach
ND, not determined.
| Gene name | WT PAP-SILAC |
G20E PAP-SILAC |
TAP |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Endo | GFP | GFP | FLAG | No. of peptides | |||||||
| Ratio H/L | No. of peptides | Ratio H/L | No. of peptides | Ratio H/L | No. of peptides | Ratio H/L | No. of peptides | Flap | WT | G20E | |
| WT-specific | |||||||||||
| MYO1D | 4.4 | 2 | 33.7 | 2 | ND | ND | |||||
| GTF3C1 | 5.4 | 6 | 8.5 | 2 | ND | ND | |||||
| PGAM5 | 9.2 | 13 | 2.8 | 1 | ND | ND | |||||
| NUDT16L1 | 3.0 | 5 | 2.6 | 8 | ND | ND | |||||
| G20E-specific | |||||||||||
| AIM1 | ND | ND | 27.4 | 48 | 4.3 | 34 | 0 | 1 | 2 | ||
| GLB1 | ND | ND | 8.8 | 5 | 2.2 | 9 | |||||
| CALU | ND | ND | 11.0 | 3 | 3.6 | 3 | |||||
| RCN1 | ND | ND | 7.5 | 6 | 3.4 | 1 | |||||
| IMMT | ND | ND | 2.3 | 15 | 5.5 | 13 | |||||
| Common | |||||||||||
| LDHA | 2.7 | 8 | 1.4 | 1 | 5.2 | 10 | 4.1 | 5 | |||
| ARF4 | 2.1 | 4 | 1.3 | 4 | 2.3 | 5 | 2.1 | 2 | |||
| YWHAQ | 2.8 | 3 | 1.0 | 6 | 2.5 | 7 | 2.1 | 5 | |||
| IQGAP1 | 3.4 | 18 | 1.2 | 50 | 2.8 | 60 | 2.8 | 5 | 0 | 1 | 5 |
| CAV1 | 6.8 | 3 | 1.5 | 4 | 4.6 | 4 | 16.4 | 9 | |||
| GNB2L1 | 3.1 | 10 | 1.3 | 2 | 2.8 | 3 | 3.6 | 2 | |||
| ANXA2 | 2.4 | 19 | 1.0 | 17 | 2.3 | 18 | 2.9 | 13 | |||
| PTEN | 11.1 | 11 | 24.2 | 32 | 39.1 | 22 | 22.8 | 24 | 0 | 87 | 98 |
| USP7 | 3.2 | 8 | 7.2 | 7 | 7.3 | 12 | 5.4 | 9 | 0 | 14 | 12 |
| TUFM | 3.4 | 8 | 2.9 | 7 | 1.9 | 11 | 3.8 | 5 | |||
| RNH1 | 3.8 | 6 | 2.2 | 11 | 2.9 | 17 | 3.5 | 4 | 3 | 10 | 4 |
| CFL1 | 3.4 | 6 | 1.7 | 14 | 2.0 | 9 | 3.1 | 6 | 2 | 6 | 6 |