FIGURE 7.

γKA-PE localizes to endoplasmic reticulum. Fluorescently labeled γKA-PE (γKA-NBD-PE, 1 μm) was incubated with HUVEC for 1 h at 4 °C and then for 15 min at 37 °C. HUVEC were fixed and immunostained with anti-calnexin antibodies to identify the endoplasmic reticulum and with DAPI to identify nuclei. Upper left panel is merged fluorescence image of γKA-NBD-PE (green, upper right panel) and anti-calnexin staining (red, lower left panel). Yellow color indicates colocalization of the γKA-NBD-PE and ER membrane. Lower right panel is nuclear (DAPI) staining of the cells. The scale bar, 5 μm.