FIGURE 6.
RGS-Linker-mediated autoinhibition of p115 DH Activity. The effects of various p115 domains on the intrinsic and the DH-catalyzed mantGDP dissociation from RhoA were measured under the same conditions as in Fig. 2. The following protein concentrations were used: 0.1 μm RhoA-mantGDP, 1 μm DH, 1 μm RGS-Linker-DH, 10 μm RGS, and 10 μm Linker. The observed rate constants (kobs) of both intrinsic and catalyzed reactions were obtained by single exponential fitting of the data. For convenience, the exact kobs values are given as numbers above the bars.