FIGURE 2.

α_vβ₃-Integrin activation mediates HAS regulation by CF. A, preincubation with the α_vβ₃-blocking antibody LM609 (5 μg/ml, 24 h) inhibited regulation of HAS isoforms by CF as determined by real time RT-PCR, B, HAS isoform expression in response to the α_vβ₃-agonist RGDS (10 μg/ml); n = 3–5, mean ± S.E., *, p < 0.05; x, not detectable. C, α_vβ₃ immunostaining of untreated and CF-treated fibroblasts at 24 h. D, actin stress fibers visualized by phalloidin staining; magnification 63×. Non-immune isotype IgG served as control.