FIGURE 1.

AGR2 and AREG protein are expressed in esophageal adenocarcinoma cells. A, serial sections of esophageal adenocarcinoma (top row) stained with hematoxylin and eosin, or immunoperoxidase after probing with AGR2 or AREG specific antisera. (second row) Immunohistochemistry of normal esophageal tissues and a protein immunoblot of JH-EsoAd1 esophageal adenocarcinoma cells to characterize the anti-AGR2 antisera. The blots consisted of WT and AGR2 RNAi KD JH-EsoAd1 cells in the absence or presence of the antigenic peptide. There is a 72-kDa cross-reactive band that does not change in the knockdown cells. B, results of staining serial sections derived from a tissue microarray containing human Barrett's esophagus and esophageal adenocarcinomas. The images may be viewed at Stanford Tissue Microarray Consortium Web Portal (array block TA-304 and TA-305). See supplement S2 for scoring of the staining intensity.